MS Committee Meeting

Sarah Tanja

2025-05-06

MS Thesis Proposal

  • proposal is complete
  • submitted signed proposal pdf to SAFS milestones

Clarifications on experiment

  • 12 treatments across leachate exposure and developmental milestones (hpf)
  • Microscopy n=9
  • July 5th & 6th
  • Originally n=10, dropped 1 cross due to no fertilization
  • Microbiome n=5 & Transcriptomics n=5
  • July 5th, 6th & 7th
  • Originally n=15, pooled samples (3 per) to have enough material to extract

Microscopy

Annotations

7 Control 4, 9, & 14 hpf

Direction of cell division from 2 to 4 proceeds from the inside out

Initial results

Microbiome

  • Partnered with mim_c for analysis
  • Shannon diversity non-significant across features

Taxa barplot

Maaslin2 significant differential features

Transcriptomics

PCA

Heatmaps

Remaining timeline

  • SP25 Finish Analyses (focus on Results section)
  • June 15th 2025 | deadline to apply for SAFS finishing fellowship for AU25
  • On-Leave status for SU25 (no funding, GI Bill runs out at the end of SP25)
  • Writing Results & Discussion SU25
  • Back again AU25
  • Sep 20th 2025 Submit Thesis Draft
  • Nov 20th 2025 Final Exam
  • Dec 12th 2025 Submit Thesis to UW